Leica DMi8 w/ Infinity TIRF from Leica Microsystems

Product Specs
ItemLeica DMi8 w/ Infinity TIRF
CompanyLeica Microsystems
Price Get Pricing Get Quote for this Product
Catalog NumberDMi8 TIRF
QuantityEA
TypeTIRF Microscope

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Leica Microsystems

10 Parkway North Blvd., Suite 300

Deerfield, Illinois 60015

United States

Phone: 800 248 0123

Company Profile

Website: www.leica-microsystems.com

(1) Purification of human β- and γ-actin from budding yeastJournal of Cell ScienceMay 1, 2023Brian K. Haarer, Morgan L. Pimm, Ebbing P. de Jong, David C. Amberg, Jessica L. Henty-Ridillafluid–air boundaries. TIRF microscopy assays We prepared and visualized TIRF imaging chambers on a DMi8 TIRF microscope (Leica Microsystems; Wetzlar, Germany) as in Henty-Ridilla (2022), with the following modifications:

(2) Structures of the TMC-1 complex illuminate mechanosensory transductionNatureOctober 27, 2022Hanbin Jeong, Sarah Clark, April Goehring, Sepehr Dehghani-Ghahnaviyeh, Ali Rasouli, Emad Tajkhorshid, Eric Gouaux5-min incubation, the slide was washed with 30 μl buffer A and the chamber was imaged using a Leica DMi8 TIRF microscope with an oil-immersion 100× objective. Images were captured using a back-illuminated EMCCD camera

(3) Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegiaBrainSeptember 14, 2022Tania Rizo et al.before recording. Images were acquired every 5 s for 10 min. TIRF recordings were conducted on a Leica DMi8 TIRF system equipped with a 100× 1.47 NA oil HC PL Apo objective and a CMOS camera (Hamamatsu) or a EMCCD camera

(4) Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sproutingiScienceMay 20, 2022Laura A. Tiemeijer, Tommaso Ristori, Oscar M.J. A. Stassen, Jaakko J. Ahlberg, Jonne J.J. de Bijl, Christopher S. Chen, Katie Bentley, Carlijn V.C. Bouten, Cecilia M. Sahlgrenactin cytoskeleton and DAPI for the nuclei respectively. Tile scan acquisition was done using a Leica DMi8 TIRF microscope. Regions of interest (ROIs) were defined as mentioned in the results and identified by visual

(5) Deafness mutation in the MYO3A motor domain impairs actin protrusion elongation mechanismMolecular Biology of the CellJanuary 1, 2022Laura K. Gunther, Joseph A. Cirilo, Rohini Desetty, Christopher M. Yengoto allow overexpression. Cells were imaged in Opti-MEM media with no phenol red with either a Leica DMi8 TIRF microscope or Leica SP8 confocal microscope equipped with a 37°C stage and objective warmer and 5% CO2

(6) Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibitionNatureJune 1, 2021Yu J, Rao P, Clark S, Mitra J, Ha T, Gouaux E.to 3 μg/mL, washed off with 30 μL of buffer A, and the chamber was immediately imaged using a Leica DMi8 TIRF microscope with an oil-immersion 100x objective. Images were captured using a back-illuminated EMCCD camera

(7) Differential regulation of local mRNA dynamics and translation following long-term potentiation and depressionProceedings of the National Academy of SciencesMarch 30, 2021Paul G. Donlin-Asp, Claudio Polisseni, Robin Klimek, Alexander Heckel, Erin M. SchumanNeurons DIV 17+ were transfected with myrVenus 12 h prior to imaging and then imaged using a Leica DMi8 TIRF microscope. A 100× oil objective (HC PL APO 100×/1.47 OIL) was used to record a field-of-view of 133

(8) Targeting zonulin and intestinal epithelial barrier function to prevent onset of arthritisNature communicationsApril 24, 2020Tajik N, Frech M, Schulz O, Schälter F, Lucas S, Azizov V, Dürholz K, Steffen F, Omata Y, Rings A, et al.three times with PBS, the sections were mounted and visualized. Imaging was performed by using the Leica DMi8 TIRF Wideﬁeld Fluorescence of ×10 objective lens (Leica, Germany) and Zeiss Spinning Disc Axio Observer Z1

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